| 1. | Reverse transcription , rt
用反转录 |
| 2. | Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus
传染性造血器官坏死病毒逆转录聚合酶链式反应操作规程 |
| 3. | Influenza virus sample in cultured cells was treated for reverse transcription , amplification , fluorescent dye labelling , hybridization and scanning
对待检样品进行随机逆转录和扩增，荧光标记，杂交，扫描分析。 |
| 4. | Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 |
| 5. | The ghr cdna was isolated from southern catfish ( silurus meridionalis ) by reverse transcription polymerase chain reaction followed by rapid amplification of cdna ends
用rt - per加race的方法从南方大口鲇中克隆出其生长激素受体( ghr ) 。 |
| 6. | The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119 , gxbm were amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) , respectively
对三株的n基因和119 、 gxbm株的g基因进行了rt - pcr扩增、克隆和测序。 |
| 7. | Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
采用cartesian基因芯片打印仪，将纯化的cdna片段打印在corning玻片表面，制作了酵母细胞基因表达谱芯片。 |
| 8. | First , mrna was purified from the total rna extracted from fresh spleens of nonimmunized mice of kunming , then the cdna library was achieved via reverse transcription pcr . gene fragments encoding v
首先从未免疫小鼠的脾脏中提取总rna ，分离出mrna ，经逆转录合成其总cdna 。 |
| 9. | The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
应用逆转录-聚合酶链式反应( rt - pcr )技术克隆得到编码草鱼生长激素( cgh )的基因cdna ，并定向克隆到puc18载体上。 |
| 10. | Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna试剂盒提取病毒rna后，逆助兴才李医‘笋脂硕圣‘老‘才转录合成cdna 。 |
